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m profile (Table 3). These effects are completely consistent with the conclusion that the 2nd ionization observed within the pH-price profile for log k
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From 3 g of mobile paste about 100 mg of homogeneous protein ended up isolated. Enzymatic activity was dependent upon the presence of disulfide decreasing reagents for instance dithiothreitol or β-mercaptoethanol during storage and purification. The indigenous molecular bodyweight with the purified enzyme was believed to be one hundred eighty kDa dependent on the elution quantity via a calibrated gel filtration column (data not revealed).
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